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Gene Card

Enter a TK1151 or OH-1004 gene ID to view sequences, annotations and analyses in the corresponding reference genome.
Flanking length, max 10000 bp
Examples TkA01G624900 LXL04_000258

Gene overview

TkA02G017280
Reference: TK1151 Chr: Chr02 Coordinates: 4063415-4065444 Strand: + Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

Download cDNA FASTA

1216 bp · TkA02G017280|TK1151|cDNA

TTCTATTTCTTTATAAGCAGCGAAGGGTTATCTGGTGGTACACAGAGAATCAAACAACCAACTACTCTCTGCTCAACAACGACAATGGCGGTAACACAAGGCAAATGGGTGAAGTTGAACCAAAAAGGAACCGGGCCCGGTGCACGAAGCTCACACGCAATCACCATTGTTGGACAAAAAGCATACGCCTTTGGTGGCGAGTTCACCCCACGCGTCCCCATCAACAACTCCCTCTACATCTTCGACCTCACCACCCAAAC...

CDS

Download CDS FASTA

981 bp · TkA02G017280|TK1151|CDS

ATGGCGGTAACACAAGGCAAATGGGTGAAGTTGAACCAAAAAGGAACCGGGCCCGGTGCACGAAGCTCACACGCAATCACCATTGTTGGACAAAAAGCATACGCCTTTGGTGGCGAGTTCACCCCACGCGTCCCCATCAACAACTCCCTCTACATCTTCGACCTCACCACCCAAACCTGGTCAACCGCCGAGTCCACAGGCGACATCCCGCCGCCACGTGTCGGCGTCACAATGGCCGCCATCGGGACCACCATCTATGT...

Protein

Download Protein FASTA

326 aa · TkA02G017280|TK1151|PROTEIN

MAVTQGKWVKLNQKGTGPGARSSHAITIVGQKAYAFGGEFTPRVPINNSLYIFDLTTQTWSTAESTGDIPPPRVGVTMAAIGTTIYVFGGRDATHTELNELYSFDTLTNEWTLLSTGDTGPTHRSYHSVATDDHRHMYVFGGCGVAGRLNDLWAYDVVENNWVEFPLPGDNLKGRGGPGLGVVGGKIWVVYGFAGMEVDDVHYFDPDGKKWVEVETTGQKPTPRSVFSSVVVGKYVFVCGGEVDPSDLGHMGAGRFTHEV...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

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ID Mapping

Input IDTkA02G017280
TK1151 IDTkA02G017280
OH-1004 IDLXL04_017662
Relationship / confidenceRbh Sequence Supported / Probable
Protein identity (%)99.000
Protein coverage (%)TK1151 95.092 / OH-1004 73.113
Coverage and evidence

Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.

Source versionTK1151 / GWHBCHF00000000
Open Full ID Mapping
SNP / Indel variants and carrier samplesCRA009020 · TK1151

Expand to load variant records for this gene.

Basic Information and Functional Annotation

Gene IDTkA02G017280
LocationChr02:4063415-4065444 (+)
DescriptionSimilar to NSP5: Nitrile-specifier protein 5
Arabidopsis / Rice Reference Genes and Papers

Download references for this gene

Arabidopsis

Reference gene IDPossible functionPapers / DOI
At3g16390 Specifier protein responsible for constitutive and herbivore-induced simple nitrile formation, especially in roots. No verified direct reference in this release
At2g33070 Specifier protein responsible for constitutive and herbivore-induced simple nitrile formation, especially in seeds.
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.2009 · DOI: 10.1074/jbc.m807500200
Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions.2012 · DOI: 10.1016/j.phytochem.2012.08.004
NSP-Dependent Simple Nitrile Formation Dominates upon Breakdown of Major Aliphatic Glucosinolates in Roots, Seeds, and Seedlings of Arabidopsis thaliana Columbia-0.2016 · DOI: 10.3389/fpls.2016.01821
Glucosinolate Content in Dormant and Germinating Arabidopsis thaliana Seeds Is Affected by Non-Functional Alleles of Classical Myrosinase and Nitrile-Specifier Protein Genes.2019 · DOI: 10.3389/fpls.2019.01549
At1g54040 Specifier protein that contributes to constitutive and herbivore-induced simple nitrile formation (By similarity). No verified direct reference in this release
At3g16400 Specifier protein responsible for constitutive and herbivore-induced simple nitrile formation, especially in seedlings and roots. No verified direct reference in this release
At3g16410 Specifier protein that contributes to constitutive and herbivore-induced simple nitrile formation. No verified direct reference in this release

Rice

No hit for this species in the current source table.

Functional annotation source
View annotation sources

Functional annotation source: OH1004 · LXL04_017662 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)
GO:0009793 embryo development ending in seed dormancy Biological Process
GO:0009845 seed germination Biological Process
GO:0010162 seed dormancy process Biological Process
GO:0019762 glucosinolate catabolic process Biological Process
GO:0080027 response to herbivore Biological Process
GO:0080028 nitrile biosynthetic process Biological Process
GO:0005634 nucleus Cellular Component
GO:0005737 cytoplasm Cellular Component
GO:0005783 endoplasmic reticulum Cellular Component
GO:0005829 cytosol Cellular Component
GO:0009506 plasmodesma Cellular Component
GO:0009507 chloroplast Cellular Component
KEGG gene cross-reference
ath:AT2G33070
ath:AT3G16390
ath:AT3G16400
ath:AT3G16410
ath:AT5G48180
These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.
Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene
Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cytoplasm0.766
Rank 1 · TkA02G017280 · TK1151
Nucleus0.472
Rank 2 · TkA02G017280 · TK1151
Peroxisome0.063
Rank 3 · TkA02G017280 · TK1151
Mitochondrion0.061
Rank 4 · TkA02G017280 · TK1151
Cell membrane0.040
Rank 5 · TkA02G017280 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Open Subcellular Module

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF24981Beta-prop_ATRN-LZTR13-81 aa
PF24681Kelch_KLHDC2_KLHL20_DRC75-32 aa
PF13415Beta-prop_FBX425-111 aa
PF13964Beta-prop_Calicin6-142 aa
PF24996NANM7-90 aa
PF13854Kelch_HCF8-93 aa
PF13418Beta-prop_TYW418-72 aa
PF01344Kelch_120-64 aa
Gene IDDomain IDDomain NameStart aaEnd aaE-valueScore
TkA02G017280PF24981Beta-prop_ATRN-LZTR13814.1e-39135.3
TkA02G017280PF24681Kelch_KLHDC2_KLHL20_DRC75327.4e-77258.3
TkA02G017280PF13415Beta-prop_FBX4251116.4e-49167.1
TkA02G017280PF13964Beta-prop_Calicin61421.3e-1248.1
TkA02G017280PF24996NANM7902.3e-0937.3
TkA02G017280PF13854Kelch_HCF8931.1e-2488.0
TkA02G017280PF13418Beta-prop_TYW418721.3e-0939.1
TkA02G017280PF01344Kelch_120641.7e-41140.0
TkA02G017280PF25210Kelch_FKB95211373.9e-1763.5
TkA02G017280PF07646Kelch_221625.6e-1245.7
TkA02G017280PF24681Kelch_KLHDC2_KLHL20_DRC7232417.4e-77258.3
TkA02G017280PF27494Y4lK26380.1213.2
TkA02G017280PF25852DUF6242_C471532.3e-0628.1
TkA02G017280PF24996NANM481432.3e-0937.3
TkA02G017280PF07893DUF166851630.3111.1
TkA02G017280PF13418Beta-prop_TYW4701621.3e-0939.1

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

Download current result

ProteinOrganismDescriptionIdentityDatabaseConfidence
O04318Arabidopsis thalianasp|O04318|JAL27_ARATH Thiohydroximate-O-sulfate sulfur/sulfate-lyase (nitrile-forming) NSP348.700%Swiss-ProtMedium
O49326Arabidopsis thalianasp|O49326|JAL21_ARATH Thiohydroximate-O-sulfate sulfur/sulfate-lyase (nitrile-forming) NSP248.900%Swiss-ProtMedium
Q8RY71Arabidopsis thalianasp|Q8RY71|ESP_ARATH N-(sulfonatooxy)alkenimidothioic acid sulfate-lyase (epithionitrile-forming)48.200%Swiss-ProtMedium
Q9SDM9Arabidopsis thalianasp|Q9SDM9|JAL28_ARATH Thiohydroximate-O-sulfate sulfur/sulfate-lyase (nitrile-forming) NSP148.300%Swiss-ProtMedium
O04316Arabidopsis thalianasp|O04316|JAL29_ARATH Thiohydroximate-O-sulfate sulfur/sulfate-lyase (nitrile-forming) NSP448.000%Swiss-ProtMedium

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