TkA08G007550 | RubberDandelionOmics

Gene overview

TkA08G007550

Reference: TK1151 Chr: Chr08 Coordinates: 1901091-1903126 Strand: + Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

1052 bp · TkA08G007550|TK1151|cDNA

TTCGGTGGACAGTTTCCCCGGTTAACACCGTTGGTGTATATTTATTTGTAGAGGAAAATTGACTAGGGTTTCTCGTCTTCCAGCGTAGCTTCAAAGCTCAACAATTCGTTTCTTCCTTCCATTCAAATCAATTCATGGGTTCCGCTTGCAAGGATGAGAGGGAGGAAGTGATTCAGGCATGGTACATGGATGATAGCAATGAAGACCAAAGGCTTCCCCATCATCGGGAACCAAAGGAATTTGTCTCTCTTGACAAACTT...

CDS

Download CDS FASTA

594 bp · TkA08G007550|TK1151|CDS

ATGGGTTCCGCTTGCAAGGATGAGAGGGAGGAAGTGATTCAGGCATGGTACATGGATGATAGCAATGAAGACCAAAGGCTTCCCCATCATCGGGAACCAAAGGAATTTGTCTCTCTTGACAAACTTGCAGAGCTCGGGGTGCTTAGCTGGCGATTAGATGCTGATAACTATGAAACTGATGAAGGATTGAAGAAAATCCGTGAAGCACGTGGATACTCTTACATGGATTTCTGTGACATTTGCCCAGAAAAGTTGCCGAA...

Protein

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197 aa · TkA08G007550|TK1151|PROTEIN

MGSACKDEREEVIQAWYMDDSNEDQRLPHHREPKEFVSLDKLAELGVLSWRLDADNYETDEGLKKIREARGYSYMDFCDICPEKLPNYEEKIKSFFEEHLHTDEEIRYCVAGSGYFDVRDAKDGWIRVWVKKGGMIVLPAGIYHRFTLDTDNYIKAMRLFVGDPIWTPFNRPHDHLPARKEYLEAFVEKGDAVDAAA

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA08G007550`
TK1151 ID	TkA08G007550
OH-1004 ID	LXL04_034353
Relationship / confidence	Rbh Sequence Supported / Probable
Protein identity (%)	100.000
Protein coverage (%)	TK1151 100.000 / OH-1004 71.636 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

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Basic Information and Functional Annotation

Gene ID TkA08G007550

Location Chr08:1901091-1903126 (+)

Description Similar to VIT_05s0020g04080: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At4g14710`	Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site.	No verified direct reference in this release
`At4g14716`	Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site.	No verified direct reference in this release
`At2g26400`	Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site.	No verified direct reference in this release

Rice

Reference gene ID	Possible function	Papers / DOI
`UniProt: A2XCT8`	Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site.	No verified direct reference in this release

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_034353 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0006555 methionine metabolic process Biological Process

GO:0019509 L-methionine salvage from methylthioadenosine Biological Process

GO:0051302 regulation of cell division Biological Process

GO:0005634 nucleus Cellular Component

GO:0005737 cytoplasm Cellular Component

GO:0005829 cytosol Cellular Component

GO:0005886 plasma membrane Cellular Component

GO:0005506 iron ion binding Molecular Function

GO:0010308 acireductone dioxygenase (Ni2+-requiring) activity Molecular Function

GO:0010309 acireductone dioxygenase [iron(II)-requiring] activity Molecular Function

GO:0016151 nickel cation binding Molecular Function

KEGG gene cross-reference

ath:AT4G14710

ath:AT4G14716

dosa:Os03g0161800

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cytoplasm0.625

Rank 1 · TkA08G007550 · TK1151

Nucleus0.411

Rank 2 · TkA08G007550 · TK1151

Cell membrane0.132

Rank 3 · TkA08G007550 · TK1151

Mitochondrion0.092

Rank 4 · TkA08G007550 · TK1151

Endoplasmic reticulum0.091

Rank 5 · TkA08G007550 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

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Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF03079ARD14-168 aa

PF02311AraC_binding94-152 aa

PF00190Cupin_194-145 aa

PF07883Cupin_296-152 aa

PF16867DMSP_lyase98-147 aa

PF07883Cupin_2158-175 aa

PF03079ARD180-191 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA08G007550	`PF03079`	ARD	14	168	3.4e-65	219.8
TkA08G007550	`PF02311`	AraC_binding	94	152	0.00023	21.6
TkA08G007550	`PF00190`	Cupin_1	94	145	0.076	13.3
TkA08G007550	`PF07883`	Cupin_2	96	152	1.2e-07	32.0
TkA08G007550	`PF16867`	DMSP_lyase	98	147	0.14	12.7
TkA08G007550	`PF07883`	Cupin_2	158	175	1.2e-07	32.0
TkA08G007550	`PF03079`	ARD	180	191	3.4e-65	219.8

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

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Protein	Organism	Description	Identity	Database	Confidence
`Q8W108`	Arabidopsis thaliana	sp\|Q8W108\|MTND3_ARATH Acireductone dioxygenase 3	82.900%	Swiss-Prot	High
`Q8GXE2`	Arabidopsis thaliana	sp\|Q8GXE2\|MTND2_ARATH Acireductone dioxygenase 2	83.900%	Swiss-Prot	High
`O48707`	Arabidopsis thaliana	sp\|O48707\|MTND1_ARATH Acireductone dioxygenase 1	78.700%	Swiss-Prot	High
`A2XCT8`	Oryza sativa subsp. indica	sp\|A2XCT8\|MTND2_ORYSI Acireductone dioxygenase 2	78.400%	Swiss-Prot	High

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