TkA05G454730 | RubberDandelionOmics

Gene overview

TkA05G454730

Reference: TK1151 Chr: Chr05 Coordinates: 113884957-113889010 Strand: - Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

1942 bp · TkA05G454730|TK1151|cDNA

AAACTCGCCGTATATTGGCATATGGTTTTTCTGCTAATAACTCTATTTATTCTCTGCTAGTGATGCCAAGGTACAAAACAAAGAAACAAAAAGGTCATATACACATATTCAATGCGGGGCGAACATTCCCTCCTAAAAACGAAAGATGATCGAATCCCAACAGTTCAACATGTGCAAATTGCGTACCATCTCTTCCAATTTCACCGCAAATCCTAATAACACGAATCTTCCCTCCTCCCAATTTCAACTCGAATCCCCCT...

CDS

Download CDS FASTA

1380 bp · TkA05G454730|TK1151|CDS

ATGTGCAAATTGCGTACCATCTCTTCCAATTTCACCGCAAATCCTAATAACACGAATCTTCCCTCCTCCCAATTTCAACTCGAATCCCCCTTTTGTTTTGGATGGAAAATTTGCTTTCCAATTGTGAAGAATCAATCGCGTTTTACTCGTTTGCTTTCCATCGACCAAGATGATCGTGTTAAAGAAACACAAATTGTGATACGGAATGGAAGAGAAGTCTTACTTCAGGCTTTCAACTGGGAGTCTCGTAAACATGATTG...

Protein

Download Protein FASTA

459 aa · TkA05G454730|TK1151|PROTEIN

MCKLRTISSNFTANPNNTNLPSSQFQLESPFCFGWKICFPIVKNQSRFTRLLSIDQDDRVKETQIVIRNGREVLLQAFNWESRKHDWWRNLERKVPDMAKCGITSAWLPPPTNSFSPEGYLPQNLYSLNSGYGSENLLKNLISKMKQYNIRPMADIVINHRVGTTQGHGGKYNRYDWIPLSWDERAVTSCTGGLGNRSTGDNFHGVPNIDHTQDFVRKDLIGWLKWLRYTVGFQDFRFDFSRGYSAKYVKEYIEGAKPIF...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA05G454730`
TK1151 ID	TkA05G454730
OH-1004 ID	LXL04_022270
Relationship / confidence	Rbh High Identity Coverage Synteny / Strict
Protein identity (%)	99.800
Protein coverage (%)	TK1151 100.000 / OH-1004 98.287 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

Expand to load variant records for this gene.

Basic Information and Functional Annotation

Gene ID TkA05G454730

Location Chr05:113884957-113889010 (-)

Description Similar to AMY2: Probable alpha-amylase 2

Arabidopsis / Rice Reference Genes and Papers

Download references for this gene

Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At1g76130`	Probable alpha-amylase that does not seem to be required for breakdown of transitory starch in leaves.	alpha-Amylase is not required for breakdown of transitory starch in Arabidopsis leaves.2005 · DOI: 10.1074/jbc.m413638200
`At1g69830`	Possesses endoamylolytic activity in vitro, but seems not required for breakdown of transitory starch in leaves.	alpha-Amylase is not required for breakdown of transitory starch in Arabidopsis leaves.2005 · DOI: 10.1074/jbc.m413638200 Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase.2008 · DOI: 10.1105/tpc.108.063487 Arabidopsis thaliana AMY3 is a unique redox-regulated chloroplastic α-amylase.2013 · DOI: 10.1074/jbc.m113.514794 Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants.2016 · DOI: 10.1105/tpc.16.00143

Rice

Reference gene ID	Possible function	Papers / DOI
`Os08g0473600`	Important for breakdown of endosperm starch during germination.	No verified direct reference in this release

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_022270 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0005975 carbohydrate metabolic process Biological Process

GO:0005983 starch catabolic process Biological Process

GO:0005987 sucrose catabolic process Biological Process

GO:0005576 extracellular region Cellular Component

GO:0005829 cytosol Cellular Component

GO:0009507 chloroplast Cellular Component

GO:0009570 chloroplast stroma Cellular Component

GO:0004556 alpha-amylase activity Molecular Function

GO:0005509 calcium ion binding Molecular Function

KEGG gene cross-reference

ath:AT1G69830

ath:AT1G76130

dosa:Os06g0713800

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Plastid0.866

Rank 1 · TkA05G454730 · TK1151

Cytoplasm0.051

Rank 2 · TkA05G454730 · TK1151

Extracellular0.049

Rank 3 · TkA05G454730 · TK1151

Mitochondrion0.039

Rank 4 · TkA05G454730 · TK1151

Lysosome / Vacuole0.036

Rank 5 · TkA05G454730 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Open Subcellular Module

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF00128Alpha-amylase94-161 aa

PF09081AMT4_dom_C132-161 aa

PF00128Alpha-amylase338-349 aa

PF09081AMT4_dom_C383-428 aa

PF00128Alpha-amylase389-405 aa

PF07821Alpha-amyl_C2403-458 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA05G454730	`PF00128`	Alpha-amylase	94	161	2.3e-09	37.7
TkA05G454730	`PF09081`	AMT4_dom_C	132	161	2e-07	31.5
TkA05G454730	`PF00128`	Alpha-amylase	338	349	2.3e-09	37.7
TkA05G454730	`PF09081`	AMT4_dom_C	383	428	2e-07	31.5
TkA05G454730	`PF00128`	Alpha-amylase	389	405	2.3e-09	37.7
TkA05G454730	`PF07821`	Alpha-amyl_C2	403	458	6.7e-22	78.3

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

Download current result

Protein	Organism	Description	Identity	Database	Confidence
`Q8LFG1`	Arabidopsis thaliana	sp\|Q8LFG1\|AMY2_ARATH Probable alpha-amylase 2	76.800%	Swiss-Prot	High
`Q94A41`	Arabidopsis thaliana	sp\|Q94A41\|AMY3_ARATH Alpha-amylase 3, chloroplastic	51.000%	Swiss-Prot	High
`P27934`	Oryza sativa subsp. japonica	sp\|P27934\|AMY3E_ORYSJ Alpha-amylase isozyme 3E	48.400%	Swiss-Prot	Medium

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