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Gene Card

Enter a TK1151 or OH-1004 gene ID to view sequences, annotations and analyses in the corresponding reference genome.
Flanking length, max 10000 bp

Gene overview

TkA03G396160
Reference: TK1151 Chr: Chr03 Coordinates: 98474540-98475188 Strand: + Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

405 bp · TkA03G396160|TK1151|cDNA

CTTGATACTGTGGGCTATAACTGGCCTCGTGAATTTGGTCAGCTCATAGCTGACATAAAAGCCAACACTCTTGGAATTGAAAATGTCATCATTTCTACTCATTGCCAGAACGATTTAGGACTTTCAACCGCCAATACTTTAGAGGGGGCTTATAATGGTGCTAGACAATTAGAAGTAACAATAAATGGAATTGGAGAGAGAGCTGGTAATGCTTCTTTGGAAGAGGTGGTGATGACTATAAAATGCAAAGGAGAGTTATT...

405 bp · TkA03G396160|TK1151|CDS

CTTGATACTGTGGGCTATAACTGGCCTCGTGAATTTGGTCAGCTCATAGCTGACATAAAAGCCAACACTCTTGGAATTGAAAATGTCATCATTTCTACTCATTGCCAGAACGATTTAGGACTTTCAACCGCCAATACTTTAGAGGGGGCTTATAATGGTGCTAGACAATTAGAAGTAACAATAAATGGAATTGGAGAGAGAGCTGGTAATGCTTCTTTGGAAGAGGTGGTGATGACTATAAAATGCAAAGGAGAGTTATT...

135 aa · TkA03G396160|TK1151|PROTEIN

LDTVGYNWPREFGQLIADIKANTLGIENVIISTHCQNDLGLSTANTLEGAYNGARQLEVTINGIGERAGNASLEEVVMTIKCKGELLGGLHTGINTRHIVMASKMVEEYSGLMVQPHKAIVGANAFAHESGIHQV

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

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ID Mapping

Input IDTkA03G396160
TK1151 IDTkA03G396160
OH-1004 IDLXL04_033194
Relationship / confidenceSequence Supported Annotation Or Model Conflict / Review
Protein identity (%)99.200
Protein coverage (%)TK1151 98.519 / OH-1004 12.102
Coverage and evidence

Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.

Source versionTK1151 / GWHBCHF00000000
SNP / Indel variants and carrier samplesCRA009020 · TK1151

Expand to load variant records for this gene.

Basic Information and Functional Annotation

Gene IDTkA03G396160
LocationChr03:98474540-98475188 (+)
DescriptionSimilar to IPMSA: 2-isopropylmalate synthase A
Arabidopsis / Rice Reference Genes and Papers

Download references for this gene

Arabidopsis

Reference gene IDPossible functionPapers / DOI
At1g74040 Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate).
At1g18500 Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate).

Rice

No hit for this species in the current source table.

Functional annotation source
View annotation sources

Functional annotation source: OH1004 · LXL04_033194 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)
GO:0009098 L-leucine biosynthetic process Biological Process
GO:0009877 nodulation Biological Process
GO:0019761 glucosinolate biosynthetic process Biological Process
GO:0009507 chloroplast Cellular Component
GO:0009570 chloroplast stroma Cellular Component
GO:0009941 chloroplast envelope Cellular Component
GO:0003852 2-isopropylmalate synthase activity Molecular Function
GO:0010177 methylthioalkylmalate synthase activity Molecular Function
GO:0016829 lyase activity Molecular Function
GO:0046872 metal ion binding Molecular Function
KEGG gene cross-reference
ath:AT1G18500
ath:AT1G74040
gmx:547778
spen:107023302
spen:107028537
These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cytoplasm0.642
Rank 1 · TkA03G396160 · TK1151
Nucleus0.296
Rank 2 · TkA03G396160 · TK1151
Mitochondrion0.116
Rank 3 · TkA03G396160 · TK1151
Cell membrane0.078
Rank 4 · TkA03G396160 · TK1151
Extracellular0.076
Rank 5 · TkA03G396160 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF00682HMGL-like2-106 aa
PF22617HCS_D237-82 aa
PF22617HCS_D2120-134 aa
Gene IDDomain IDDomain NameStart aaEnd aaE-valueScore
TkA03G396160PF00682HMGL-like21065.2e-29102.1
TkA03G396160PF22617HCS_D237820.0001522.8
TkA03G396160PF22617HCS_D21201340.0001522.8

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

Download current result

ProteinOrganismDescriptionIdentityDatabaseConfidence
Q9C550Arabidopsis thalianasp|Q9C550|LEU12_ARATH 2-isopropylmalate synthase 2, chloroplastic83.600%Swiss-ProtHigh
Q9LPR4Arabidopsis thalianasp|Q9LPR4|LEU11_ARATH 2-isopropylmalate synthase 1, chloroplastic82.100%Swiss-ProtHigh

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