TkA02G448670 | RubberDandelionOmics

Gene overview

TkA02G448670

Reference: TK1151 Chr: Chr02 Coordinates: 114100871-114103597 Strand: + Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

1502 bp · TkA02G448670|TK1151|cDNA

CAACATCAATCATTGCAATTCAAAGAAAGATTTCAGGACTTACACCAAAAGAAGCCGTGGAGAACTGGAGATAGGAGACTATGGCGATGAACTCCGATTATGGAGCTTTCATGGAGAAATTCATCCTCTCTCCGCTGCCAACCACTTCTCCTGCGCAGCTCCCGTTGAATGGTCTCACTTTTGCAGTCAAAGACATATTTGACATGGAAGGACACGTGAGCGGCTTTGGGAATCCAGATTGGTTAAAAACTCATTCTGCT...

CDS

Download CDS FASTA

1458 bp · TkA02G448670|TK1151|CDS

CAACATCAATCATTGCAATTCAAAGAAAGATTTCAGGACTTACACCAAAAGAAGCCGTGGAGAACTGGAGATAGGGTACCCACCTTATACTTCACTCCTATTTTATCTTCTTTAACTGAAAATTCTCCACATACAGAGACTATGGCGATGAACTCCGATTATGGAGCTTTCATGGAGAAATTCATCCTCTCTCCGCTGCCAACCACTTCTCCTGCGCAGCTCCCGTTGAATGGTCTCACTTTTGCAGTCAAAGACATATT...

Protein

Download Protein FASTA

485 aa · TkA02G448670|TK1151|PROTEIN

QHQSLQFKERFQDLHQKKPWRTGDRVPTLYFTPILSSLTENSPHTETMAMNSDYGAFMEKFILSPLPTTSPAQLPLNGLTFAVKDIFDMEGHVSGFGNPDWLKTHSAATSTSPAVLAMLKAGATCVGRTIMDEMAYSINGENIHYGTPINPCAPDRVPGGSSSGSSVAVSANLADFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTQGVIPMAQSFDTVGWFCRDPGTLNRVGRILLGLPNEKLVGPTKVFIAEDCFKL...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA02G448670`
TK1151 ID	TkA02G448670
OH-1004 ID	LXL04_014185
Relationship / confidence	Rbh High Identity Coverage Synteny / Strict
Protein identity (%)	96.300
Protein coverage (%)	TK1151 100.000 / OH-1004 96.627 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

Expand to load variant records for this gene.

Basic Information and Functional Annotation

Gene ID TkA02G448670

Location Chr02:114100871-114103597 (+)

Description Similar to AMI1: Amidase 1

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At1g08980`	Amidase involved in auxin biosynthesis.	Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid.2003 · DOI: 10.1016/s0031-9422(02)00563-0 Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis.2014 · DOI: 10.3390/plants3030324
`At3g17970`	Chaperone receptor mediating Hsp90-dependent protein targeting to chloroplasts.	Toc64/OEP64 is not essential for the efficient import of proteins into chloroplasts in Arabidopsis thaliana.2007 · DOI: 10.1111/j.1365-313x.2007.03207.x
`At5g09420`	Chaperone receptor mediating Hsp90-dependent protein targeting to mitochondria.	No verified direct reference in this release

Rice

Reference gene ID	Possible function	Papers / DOI
`Os04g0118100`	Amidase involved in auxin biosynthesis (Probable).	Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis.2014 · DOI: 10.3390/plants3030324

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_014185 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0006626 protein targeting to mitochondrion Biological Process

GO:0009684 indoleacetic acid biosynthetic process Biological Process

GO:0009851 auxin biosynthetic process Biological Process

GO:0015031 protein transport Biological Process

GO:0030150 protein import into mitochondrial matrix Biological Process

GO:0005576 extracellular region Cellular Component

GO:0005654 nucleoplasm Cellular Component

GO:0005737 cytoplasm Cellular Component

GO:0005739 mitochondrion Cellular Component

GO:0005741 mitochondrial outer membrane Cellular Component

GO:0005829 cytosol Cellular Component

GO:0009507 chloroplast Cellular Component

KEGG gene cross-reference

ath:AT1G08980

ath:AT3G17970

ath:AT5G09420

dosa:Os04g0118100

osa:4334958

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cytoplasm0.525

Rank 1 · TkA02G448670 · TK1151

Nucleus0.210

Rank 2 · TkA02G448670 · TK1151

Mitochondrion0.184

Rank 3 · TkA02G448670 · TK1151

Peroxisome0.151

Rank 4 · TkA02G448670 · TK1151

Cell membrane0.074

Rank 5 · TkA02G448670 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

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Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF01425Amidase72-226 aa

PF02522Antibiotic_NAT366-401 aa

PF01425Amidase376-463 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA02G448670	`PF01425`	Amidase	72	226	7.5e-56	190.8
TkA02G448670	`PF02522`	Antibiotic_NAT	366	401	0.17	12.1
TkA02G448670	`PF01425`	Amidase	376	463	7.5e-56	190.8

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

Download current result

Protein	Organism	Description	Identity	Database	Confidence
`Q9FR37`	Arabidopsis thaliana	sp\|Q9FR37\|AMI1_ARATH Amidase 1	63.600%	Swiss-Prot	High
`Q9LVH5`	Arabidopsis thaliana	sp\|Q9LVH5\|OE64C_ARATH Outer envelope protein 64, chloroplastic	48.100%	Swiss-Prot	Medium
`F4KCL7`	Arabidopsis thaliana	sp\|F4KCL7\|OE64M_ARATH Outer envelope protein 64, mitochondrial	47.600%	Swiss-Prot	Medium
`Q7XTK3`	Oryza sativa subsp. japonica	sp\|Q7XTK3\|AMI1_ORYSJ Amidase 1	59.800%	Swiss-Prot	High

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