Rubber Biosynthesis Focus

A pathway-centered entry for MVA/MEP, IPP/DMAPP, prenyl donors, CPT/CPTL, REF/SRPP, latex/root-associated and terpenoid candidate genes.

This topic is built from precomputed GFF/GO/KEGG/homology candidate sets and linked evidence from expression, proteome, localization, Pfam, Swiss-Prot, PPI, coexpression and TFBS modules. Results are candidate evidence, not experimental validation.

The current database contains 5523 candidate records, 4979 candidate genes, and 8 pathway steps; the main display is centered on the TK1151 reference while OH-1004/LXL04 remains queryable and linked.

Examples TkA01G624900 LXL04_000258

Download current filtered candidate TSV

Cross-species Research Reference

4 reference genes are counted separately from rubber-dandelion candidates. They are literature, omics, or sequence resources from Para rubber tree, guayule, a related dandelion, and Eucommia; external function or homology is not direct validation in rubber dandelion.

1. Choose a speciesClick a species card or use the filter to focus on one reference species and material context.

2. Read the evidence typeRead the evidence and boundary column: direct experiments, omics associations, and sequence resources have different interpretation ranges.

3. Verify the sourceEach DOI, article, or public database entry is clickable; download the current filter for further comparison.

Eucommia records are a trans-polyisoprene comparator only and are excluded from natural cis-rubber candidate and pathway-card counts.

4Para rubber treeClick to view · 2 sources · cis-1,4-polyisoprene

Download current cross-species reference TSV

Species and material	Reference gene / sequence ID	Pathway step	Evidence and boundary	Source
Para rubber treeHevea brasiliensiscis-1,4-polyisopreneRubber particles	`HRBP`LC057267CPTL	CPT/CPTL branchHRT1-complex helper subunit	A_direct_experiment HRBP was reported as a helper subunit in the HRT1 rubber-particle complex. Complex-support evidence, not a stand-alone catalytic claim.	10.7554/eLife.19022Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis
Para rubber treeHevea brasiliensiscis-1,4-polyisopreneRubber particles	`HRT1`BAB71776.2;AB061234.3CPT	CPT/CPTL branchRubber-particle cis-prenyltransferase	A_direct_experiment HRT1 showed in-vitro rubber-transferase activity on detergent-washed rubber particles, with higher activity for the HRT1-REF-HRBP complex. Direct evidence in Hevea rubber particles; not direct validation in rubber dandelion.	10.7554/eLife.19022Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis
Para rubber treeHevea brasiliensiscis-1,4-polyisopreneLatex and recombinant enzyme	`HRT2`Q8W3U4;AB064661.2CPT	CPT/CPTL branchLatex-expressed cis-prenyltransferase reference	A_enzyme_experiment HRT2 is retained as a cis-prenyltransferase and latex-expression reference. Do not claim direct reconstitution of high-molecular-weight natural rubber by HRT2 alone.	10.1046/j.1432-1033.2003.03863.xMolecular cloning expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis
Para rubber treeHevea brasiliensiscis-1,4-polyisopreneRubber particles	`REF`REF complex memberREF	REF/SRPP rubber-particle proteinsRubber-particle complex member	A_complex_evidence HRT1 and HRBP/REF particle-complex evidence; REF is not displayed as a stand-alone catalytic proof. Not a stand-alone catalytic proof.	10.7554/eLife.19022Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis

Para rubber tree · HRBP

Sequence IDLC057267

StepCPT/CPTL branch · CPTL

EvidenceHRBP was reported as a helper subunit in the HRT1 rubber-particle complex.

BoundaryComplex-support evidence, not a stand-alone catalytic claim.

Source10.7554/eLife.19022

Para rubber tree · HRT1

Sequence IDBAB71776.2;AB061234.3

StepCPT/CPTL branch · CPT

EvidenceHRT1 showed in-vitro rubber-transferase activity on detergent-washed rubber particles, with higher activity for the HRT1-REF-HRBP complex.

BoundaryDirect evidence in Hevea rubber particles; not direct validation in rubber dandelion.

Source10.7554/eLife.19022

Para rubber tree · HRT2

Sequence IDQ8W3U4;AB064661.2

StepCPT/CPTL branch · CPT

EvidenceHRT2 is retained as a cis-prenyltransferase and latex-expression reference.

BoundaryDo not claim direct reconstitution of high-molecular-weight natural rubber by HRT2 alone.

Source10.1046/j.1432-1033.2003.03863.x

Para rubber tree · REF

Sequence IDREF complex member

StepREF/SRPP rubber-particle proteins · REF

EvidenceHRT1 and HRBP/REF particle-complex evidence; REF is not displayed as a stand-alone catalytic proof.

BoundaryNot a stand-alone catalytic proof.

Source10.7554/eLife.19022

Pathway Navigation And Candidate Distribution

MVA · Mevalonate pathway

High 40 · Medium 3 · Low 4 MEP · MEP pathway

High 23 · Medium 0 · Low 36 IPP/DMAPP/FPP · Prenyl-donor metabolism

271

High 141 · Medium 83 · Low 47 CPT/CPTL · Cis-prenyltransferase branch

High 0 · Medium 0 · Low 5 REF/SRPP · Rubber-particle proteins

High 0 · Medium 0 · Low 7 Rubber · Rubber biosynthesis candidates

288

High 1 · Medium 287 · Low 0 Terpenoid · Terpenoid metabolism

380

High 72 · Medium 107 · Low 201 Latex/root · Latex/root associated candidates

4466

High 231 · Medium 1284 · Low 2951

377 MVA/MEP and isoprenoid

Click to filter · High 204 · Medium 86 · Low 87 4466 Latex/root related candidates

Click to filter · High 231 · Medium 1284 · Low 2951 300 Rubber biosynthesis

Click to filter · High 1 · Medium 287 · Low 12 380 Terpenoid metabolism

Click to filter · High 72 · Medium 107 · Low 201

Candidate Genes

300 candidates match the current filter; 10 are shown per page (page 11 of 30). TSV exports up to 2000 rows under the current filters. Use pathway nodes or summary cards to filter, then inspect Gene Card and linked modules for evidence details.

Gene ID	Pathway/step	Confidence	Evidence layers	Location	Key evidence	Actions
`LXL04_015620` TK1151: - OH-1004: LXL04_015620	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:77809116-77812009 +	GO:0016114	Gene Card Expression Proteome JBrowse
`TkA01G321560` TK1151: TkA01G321560;TkA01G321570;TkA01G321580 OH-1004: LXL04_015867	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtPPICoexpression	CM104801.1:93453094-93459948 -	GO:0016740	Gene Card Expression Proteome JBrowse
`TkA02G232460` TK1151: TkA02G232460 OH-1004: LXL04_015881	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtPPICoexpression	CM104801.1:94589480-94590773 -	GO:0016114	Gene Card Expression Proteome JBrowse
`TkA02G180040` TK1151: TkA02G180040 OH-1004: LXL04_016178	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:113362366-113364045 -	GO:0016114	Gene Card Expression Proteome JBrowse
`LXL04_016180` TK1151: - OH-1004: LXL04_016180	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:113396600-113398279 -	GO:0016114	Gene Card Expression Proteome JBrowse
`TkA02G159610` TK1151: TkA02G159610;TkA02G159720 OH-1004: LXL04_016299	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationSwiss-ProtCoexpression	CM104801.1:117805721-117806576 +	GO:0008299	Gene Card Expression Proteome JBrowse
`LXL04_016597` TK1151: - OH-1004: LXL04_016597	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:128406027-128408717 -	GO:0016114	Gene Card Expression Proteome JBrowse
`LXL04_016664` TK1151: - OH-1004: LXL04_016664	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtPPICoexpression	CM104801.1:130385702-130391884 +	GO:0008299	Gene Card Expression Proteome JBrowse
`TkA02G065950` TK1151: TkA02G065950;TkA02G066110;TkA02G066200;TkA02G066460 OH-1004: LXL04_017217	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:143835022-143836683 +	GO:0016114	Gene Card Expression Proteome JBrowse
`TkA02G065710` TK1151: TkA02G065710;TkA02G402640 OH-1004: LXL04_017221	Rubber biosynthesis Rubber biosynthesis candidates	Medium score 6	GOExpressionLocalizationPfamSwiss-ProtCoexpression	CM104801.1:143928858-143934585 +	GO:0016114	Gene Card Expression Proteome JBrowse

`LXL04_015620`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source ID-

LocationCM104801.1:77809116-77812009 +

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`TkA01G321560`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA01G321560;TkA01G321570;TkA01G321580

LocationCM104801.1:93453094-93459948 -

EvidenceGOExpressionLocalizationPfamSwiss-ProtPPICoexpression

Key evidenceGO:0016740

Gene Card Expression Proteome JBrowse

`TkA02G232460`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA02G232460

LocationCM104801.1:94589480-94590773 -

EvidenceGOExpressionLocalizationPfamSwiss-ProtPPICoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`TkA02G180040`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA02G180040

LocationCM104801.1:113362366-113364045 -

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`LXL04_016180`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source ID-

LocationCM104801.1:113396600-113398279 -

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`TkA02G159610`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA02G159610;TkA02G159720

LocationCM104801.1:117805721-117806576 +

EvidenceGOExpressionLocalizationSwiss-ProtCoexpression

Key evidenceGO:0008299

Gene Card Expression Proteome JBrowse

`LXL04_016597`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source ID-

LocationCM104801.1:128406027-128408717 -

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`LXL04_016664`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source ID-

LocationCM104801.1:130385702-130391884 +

EvidenceGOExpressionLocalizationPfamSwiss-ProtPPICoexpression

Key evidenceGO:0008299

Gene Card Expression Proteome JBrowse

`TkA02G065950`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA02G065950;TkA02G066110;TkA02G066200;TkA02G066460

LocationCM104801.1:143835022-143836683 +

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

`TkA02G065710`

Rubber biosynthesisRubber biosynthesis candidatesMedium · score 6

Source IDTkA02G065710;TkA02G402640

LocationCM104801.1:143928858-143934585 +

EvidenceGOExpressionLocalizationPfamSwiss-ProtCoexpression

Key evidenceGO:0016114

Gene Card Expression Proteome JBrowse

Evidence Boundary

Current results integrate annotation, GO/KEGG, homology, predicted localization, domains, expression/proteome and network evidence. High/Medium/Low indicates evidence density and consistency, not experimental validation.