GO / KEGG Statistical Enrichment
Over-representation analysis against explicit native-gene backgrounds. Gene annotations and statistical results are shown separately.
The example contains 12 candidate terpenoid-backbone pathway genes from this annotation release, for demonstration. TK1151 and OH1004 are analyzed independently, without cross-material mapping or gene merging.
The default background consists of native genes annotated in the selected GO aspect or KEGG pathway collection. A custom background is intersected with that same annotation universe. Missing annotation does not mean missing function; source coverage limits this analysis.
Input and annotation coverage
TK1151 native genes: 45224 · Annotation release: native_plant_ORA_20261013
Gene-level GO and KEGG annotations
One row per native gene; GO includes is_a/part_of ancestors. Up to 50 genes are shown; downloads include all genes. All annotations are computational homolog transfers.
| Native gene ID | GO | KEGG pathways / KO | Donor protein / Identity / Query coverage |
|---|---|---|---|
| TkA01G624900 | GO:0003824 catalytic activity; GO:0004419 hydroxymethylglutaryl-CoA lyase activity; GO:0005488 binding; GO:0005622 intracellular anatomical structure; GO:0005737 cytoplasm; GO:0005739 mitochondrion; GO:0005759 mitochondrial matrix; GO:0006520 amino acid metabolic process; GO:0006551 L-leucine metabolic process; GO:0006552 L-leucine catabolic process; GO:0006629 lipid metabolic process; GO:0008152 metabolic process; GO:0008610 lipid biosynthetic process; GO:0009056 catabolic process; GO:0009058 biosynthetic process; GO:0009063 amino acid catabolic process; GO:0009081 branched-chain amino acid metabolic process; GO:0009083 branched-chain amino acid catabolic process; GO:0009507 chloroplast; GO:0009536 plastid; GO:0009987 cellular process; GO:0016829 lyase activity; GO:0016830 carbon-carbon lyase activity; GO:0016833 oxo-acid-lyase activity; GO:0019752 carboxylic acid metabolic process; GO:0031974 membrane-enclosed lumen; GO:0036094 small molecule binding; GO:0043167 ion binding; GO:0043169 cation binding; GO:0043226 organelle; GO:0043227 membrane-bounded organelle; GO:0043229 intracellular organelle; GO:0043231 intracellular membrane-bounded organelle; GO:0043233 organelle lumen; GO:0043436 oxoacid metabolic process; GO:0044238 primary metabolic process; GO:0044281 small molecule metabolic process; GO:0044282 small molecule catabolic process; GO:0044283 small molecule biosynthetic process; GO:0046395 carboxylic acid catabolic process; GO:0046872 metal ion binding; GO:0046951 ketone body biosynthetic process; GO:0070013 intracellular organelle lumen; GO:0110165 cellular anatomical structure; GO:0170039 proteinogenic amino acid metabolic process; GO:0170040 proteinogenic amino acid catabolic process; GO:1901568 fatty acid derivative metabolic process; GO:1901570 fatty acid derivative biosynthetic process | ath00280 Valine, leucine and isoleucine degradation; ath00650 Butanoate metabolism; ath01100 Metabolic pathways; ath04146 Peroxisome K01640 | O81027 72.0 / 77.59 |
Statistical enrichment results
One-sided hypergeometric test; Benjamini–Hochberg correction separately for BP, MF, CC and KEGG. Every term with 2–5,000 background genes participates, including zero-overlap terms. The table reports terms with overlap, including nonsignificant terms. The display threshold is FDR ≤ 0.05.
| Group | N eligible background | n eligible foreground | Tested terms | FDR≤0.05 | Foreground genes excluded |
|---|---|---|---|---|---|
| biological_process | 11100 | 1 | 3880 | 0 | 0 |
Both background and foreground count distinct native genes. N/n may differ across groups because annotation coverage differs. FDR is interpreted within each reported test family.
Terms with overlap: 21 · Page 1/1 · 50 rows per page; complete download
| ID / Name | Group | k/n | K/N | Expected | Fold enrichment | P | FDR (BH) | Overlap genes |
|---|---|---|---|---|---|---|---|---|
| GO:0046951 ketone body biosynthetic process | biological_process | 1/1 | 3/11100 | 0.00027 | 3.7e+03 | 0.00027 | 1 | TkA01G624900 |
| GO:0006552 L-leucine catabolic process | biological_process | 1/1 | 11/11100 | 0.000991 | 1.01e+03 | 0.000991 | 1 | TkA01G624900 |
| GO:0006551 L-leucine metabolic process | biological_process | 1/1 | 16/11100 | 0.00144 | 694 | 0.00144 | 1 | TkA01G624900 |
| GO:0009083 branched-chain amino acid catabolic process | biological_process | 1/1 | 24/11100 | 0.00216 | 462 | 0.00216 | 1 | TkA01G624900 |
| GO:0009081 branched-chain amino acid metabolic process | biological_process | 1/1 | 37/11100 | 0.00333 | 300 | 0.00333 | 1 | TkA01G624900 |
| GO:0170040 proteinogenic amino acid catabolic process | biological_process | 1/1 | 50/11100 | 0.0045 | 222 | 0.0045 | 1 | TkA01G624900 |
| GO:1901570 fatty acid derivative biosynthetic process | biological_process | 1/1 | 53/11100 | 0.00477 | 209 | 0.00477 | 1 | TkA01G624900 |
| GO:0009063 amino acid catabolic process | biological_process | 1/1 | 57/11100 | 0.00514 | 195 | 0.00514 | 1 | TkA01G624900 |
| GO:1901568 fatty acid derivative metabolic process | biological_process | 1/1 | 66/11100 | 0.00595 | 168 | 0.00595 | 1 | TkA01G624900 |
| GO:0046395 carboxylic acid catabolic process | biological_process | 1/1 | 111/11100 | 0.01 | 100 | 0.01 | 1 | TkA01G624900 |
| GO:0170039 proteinogenic amino acid metabolic process | biological_process | 1/1 | 138/11100 | 0.0124 | 80.4 | 0.0124 | 1 | TkA01G624900 |
| GO:0044282 small molecule catabolic process | biological_process | 1/1 | 182/11100 | 0.0164 | 61 | 0.0164 | 1 | TkA01G624900 |
| GO:0006520 amino acid metabolic process | biological_process | 1/1 | 230/11100 | 0.0207 | 48.3 | 0.0207 | 1 | TkA01G624900 |
| GO:0044283 small molecule biosynthetic process | biological_process | 1/1 | 439/11100 | 0.0395 | 25.3 | 0.0395 | 1 | TkA01G624900 |
| GO:0008610 lipid biosynthetic process | biological_process | 1/1 | 462/11100 | 0.0416 | 24 | 0.0416 | 1 | TkA01G624900 |
| GO:0019752 carboxylic acid metabolic process | biological_process | 1/1 | 551/11100 | 0.0496 | 20.1 | 0.0496 | 1 | TkA01G624900 |
| GO:0043436 oxoacid metabolic process | biological_process | 1/1 | 600/11100 | 0.0541 | 18.5 | 0.0541 | 1 | TkA01G624900 |
| GO:0006629 lipid metabolic process | biological_process | 1/1 | 761/11100 | 0.0686 | 14.6 | 0.0686 | 1 | TkA01G624900 |
| GO:0044281 small molecule metabolic process | biological_process | 1/1 | 1043/11100 | 0.094 | 10.6 | 0.094 | 1 | TkA01G624900 |
| GO:0009056 catabolic process | biological_process | 1/1 | 1124/11100 | 0.101 | 9.88 | 0.101 | 1 | TkA01G624900 |
| GO:0009058 biosynthetic process | biological_process | 1/1 | 2662/11100 | 0.24 | 4.17 | 0.24 | 1 | TkA01G624900 |
Enrichment plots
Top 10 overlapping terms ranked by FDR; nonsignificant terms may be included. Bubble area increases with overlap count; k is labeled. Plot −log10 FDR is capped at 300; original log P/FDR are retained in downloads.
On small screens, scroll horizontally to inspect the full plot at a readable text size.
Fold enrichment and overlap count
Adjusted significance
Methods, sources and interpretation
Independent TK1151/OH1004 native proteins; overall top-ranked reviewed Arabidopsis homolog only, identity >=30%, query coverage >=70%, E<=1e-5; homology transfer is not orthology or experimental validation. Conservative source coverage, not a full functional annotation.
Homology transfer does not establish orthology or experimentally validated function in this species. Enrichment identifies statistical over-representation in the current annotation set, not regulation, pathway activation or causality. Use the experimentally detectable gene background for transcriptomic or proteomic analyses.
GO data-version: releases/2026-07-26 · Excluded animal-related KEGG maps: ath04820, ath04620, ath04382, ath05100
Source files and SHA-256
ath_pathways.tsv · 2026-10-13T20:39:33+0800bdc8ed90c67e6f739fe1fef0742fff200d6dcbe264765a9070dff56bacf0b5ee
ath_gene_pathways.tsv · 2026-10-13T20:39:34+08006a2f07a143ecbc09b16c54ded5cb14e033cbe78eead30154c5bacb00ce9a5aea
ath_gene_ko.tsv · 2026-10-13T20:39:35+0800c408988440e9bed09f2d7ce3e6b4c7f0daf04167111344ba326263bc3f33c9d7
kegg_info.txt · 2026-10-13T20:39:36+080081e6c1b68c0afe91233190118bc0e94464b9f5c33114e3794e3b060b7975b0fb
ath_reviewed_go_kegg.tsv · 2026-10-13T20:48:07+0800684cb804a34ec241933dc67b2d1b9f0dc9a65332b92e8330f31ac28d927d2070
go-basic.obo · 2026-10-13T20:50:35+0800b08d45b268b8c24ccb2513dbbbc7d4df9f6521c099b413f79eb31e06e0fa3bcc
Taraxacum taxon-filter SHA-256: 6e0c25c39c237c8e9223174f09420ac21f12b9f6ae5bccc9f431337f3da3fc5c