query	gene_id	record_type	id	name	namespace_or_position	evidence	source	query_reference	annotation_reference	annotation_gene_id	annotation_assembly	mapping_category	mapped_annotation
TkA03G220440	TkA03G220440	function	TkA03G220440	Similar to HAP2: Protein HAPLESS 2 (Arabidopsis thaliana OX%3D3702)		GFF-derived	GCA_047496595.1_OSU_TarKok_2.0	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0007338	single fertilization	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=3; identity=23.675; coverage=86.286; evalue=2.22e-46; UniProt GO evidence=IMP:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0007342	fusion of sperm to egg plasma membrane involved in single fertilization	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=4; identity=24.398; coverage=94.857; evalue=2.79e-42; UniProt GO evidence=IMP:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0009566	fertilization	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=3; identity=23.675; coverage=86.286; evalue=2.22e-46; UniProt GO evidence=IMP:GO_Central	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0009567	double fertilization forming a zygote and endosperm	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IMP:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0010183	pollen tube guidance	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IMP:TAIR	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0045026	plasma membrane fusion	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0048235	pollen sperm cell differentiation	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IEP:TAIR	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0051205	protein insertion into membrane	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=4; identity=24.398; coverage=94.857; evalue=2.79e-42; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0061936	fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm	biological_process	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IMP:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0005576	extracellular region	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=ISM:TAIR	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0005783	endoplasmic reticulum	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IDA:TAIR	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0005789	endoplasmic reticulum membrane	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IEA:UniProtKB-SubCell	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0005886	plasma membrane	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0005911	cell-cell junction	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=3; identity=23.675; coverage=86.286; evalue=2.22e-46; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0030659	cytoplasmic vesicle membrane	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=4; identity=24.398; coverage=94.857; evalue=2.79e-42; UniProt GO evidence=IEA:UniProtKB-SubCell	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0031253	cell projection membrane	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=4; identity=24.398; coverage=94.857; evalue=2.79e-42; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0031410	cytoplasmic vesicle	cellular_component	Swiss-Prot reviewed homolog transfer; hit_rank=4; identity=24.398; coverage=94.857; evalue=2.79e-42; UniProt GO evidence=IDA:UniProtKB	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0008289	lipid binding	molecular_function	Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0; UniProt GO evidence=IEA:UniProtKB-KW	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	GO	GO:0140522	fusogenic activity	molecular_function	Swiss-Prot reviewed homolog transfer; hit_rank=3; identity=23.675; coverage=86.286; evalue=2.22e-46; UniProt GO evidence=IDA:GO_Central	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	KEGG	ath:AT4G11720	KEGG gene cross-reference from reviewed UniProt hit; pathway name pending		Swiss-Prot reviewed homolog transfer; hit_rank=1; identity=59.452; coverage=99.0; evalue=0.0	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	KEGG	dosa:Os09g0525700	KEGG gene cross-reference from reviewed UniProt hit; pathway name pending		Swiss-Prot reviewed homolog transfer; hit_rank=2; identity=55.363; coverage=82.571; evalue=0.0	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	KEGG	nve:5507857	KEGG gene cross-reference from reviewed UniProt hit; pathway name pending		Swiss-Prot reviewed homolog transfer; hit_rank=5; identity=26.99; coverage=73.571; evalue=4.58e-40	UniProtKB/Swiss-Prot DAT 2026 current_release	TK1151	OH1004	LXL04_026479	GCA_047496595.1_OSU_TarKok_2.0	many_to_one	True
TkA03G220440	TkA03G220440	organism_homology	F4JP36	sp|F4JP36|HAP2_ARATH Protein HAPLESS 2	Arabidopsis thaliana	identity=51.6; coverage=56.886; evalue=2.51e-31; rank=1	TK1151 Swiss-Prot homology; source_gene=TkA03G220440; source_db=UniProt Swiss-Prot local database (DIAMOND)	TK1151	TK1151	TkA03G220440			False
TkA03G220440	TkA03G220440	organism_homology	Q5VNM3	sp|Q5VNM3|IF43A_ORYSJ Eukaryotic initiation factor 4A-III homolog A	Oryza sativa subsp. japonica	identity=78.1; coverage=38.323; evalue=1.29e-23; rank=3	TK1151 Swiss-Prot homology; source_gene=TkA03G220440; source_db=UniProt Swiss-Prot local database (DIAMOND)	TK1151	TK1151	TkA03G220440			False
TkA03G220440	TkA03G220440	reference_gene_literature	At4g11720	男性生育所需。	Arabidopsis	Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization.; PMID=17079265; DOI=10.1242/dev.02683	https://pubmed.ncbi.nlm.nih.gov/17079265/						
TkA03G220440	TkA03G220440	reference_gene_literature	At4g11720	男性生育所需。	Arabidopsis	HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain.; PMID=20333238; DOI=10.1371/journal.pgen.1000882	https://pubmed.ncbi.nlm.nih.gov/20333238/						
TkA03G220440	TkA03G220440	reference_gene_literature	At4g11720	男性生育所需。	Arabidopsis	The functional domain of GCS1-based gamete fusion resides in the amino terminus in plant and parasite species.; PMID=21209845; DOI=10.1371/journal.pone.0015957	https://pubmed.ncbi.nlm.nih.gov/21209845/						
TkA03G220440	TkA03G220440	reference_gene_literature	At4g11720	男性生育所需。	Arabidopsis	Egg cell-secreted EC1 triggers sperm cell activation during double fertilization.; PMID=23180860; DOI=10.1126/science.1223944	https://pubmed.ncbi.nlm.nih.gov/23180860/						
TkA03G220440	TkA03G220440	reference_gene_literature	At4g11720	男性生育所需。	Arabidopsis	 Arabidopsis  HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens.; PMID=28137780; DOI=10.1083/jcb.201610093	https://pubmed.ncbi.nlm.nih.gov/28137780/						
TkA03G220440	TkA03G220440	reference_gene_function	At3g19760	ATP依赖的RNA解旋酶。	Arabidopsis	暂无已核实直接文献							
TkA03G220440	TkA03G220440	reference_gene_function	Os01g0639100	ATP依赖的RNA解旋酶。	Rice	暂无已核实直接文献							
TkA03G220440	TkA03G220440	reference_gene_literature	Os03g0566800	ATP依赖的RNA解旋酶。	Rice	Two highly similar DEAD box proteins, OsRH2 and OsRH34, homologous to eukaryotic initiation factor 4AIII, play roles of the exon junction complex in regulating growth and development in rice.; PMID=27071313; DOI=10.1186/s12870-016-0769-5	https://pubmed.ncbi.nlm.nih.gov/27071313/						
